In conclusion, an amide-containing adenine-mediated zinc-based rod MOF of 1 was successfully constructed by virtue of solvothermal reaction and mixed-linker approach. SCXRD discloses that 1 features no open meal sites and exhibits 1D microporous channels replete with uncoordinated carboxylate oxygen atoms and amide groups, resulting in not only C₂H₂ but also C₂H₆ selective property. Therefore, the isolated single material of 1 is capable of separating C₂H₄ from a ternary mixture of C₂H₂/C₂H₄/C₂H₆ in one step, which was ultimately confirmed by dynamic breakthrough experiments. This work provides an impressive multifunctional MOF material toward the challenging one-step ethylene purification application.