A novel Schiff-base fluorescent probe 6,6΄-((1E,1΄E)-(ethane-1,2-diylbis(azaneylylidene))bis-  (methaneylylidene))bis(3-(diethylamino)phenol) (L) was derived from the 2:1 M condensation of 4-(diethylamino)- 2-hydroxybenzaldehyde with ethylenediamine and characterized by ¹H NMR, ¹³C NMR and FT-IR spectroscopies. The results of spectral analysis showed that the probe L is selective and sensitive to Cu²⁺. The detection limit of L is found to be 19 nmol·L^-1. There is a good linear relationship between the fluorescence intensity of probe L and the concentration of Cu²⁺ in the range of 0 to 20 μmol×L^-1. X-ray crystal structure of the L-Cu²⁺ complex and the Job plot revealed a 1:1 L-Cu²⁺ identification.