A highly selective iron ions fluorescent probe based on the benz(c)acridine-1,2,3-triazole derivatives was produced by multi-step reactions. 1-(7-Benz[c]acridinyl)-4-(4-methylphenyl)-1,2,3-triazole (4a), C₂₆H₁₈N₄, was structurally determined by single-crystal X-ray diffraction. It crystallizes in the trigonal system, space group R-3 with a = 36.230(10), b = 36.230(10), c = 7.993(3) Å, β = 90°, V = 9086(6) Å³, Z = 18, D_c = 1.271 g/cm³, F(000) = 3636, μ = 0.602 mm^-1, the final R = 0.0865 and wR = 0.1619 for 3951 observed reflections (I > 2σ(I)). X-ray analysis indicated that all four rings of benz(c)acridine are in the same plane, and the 1,2,3-triazole ring and the corresponding linked benzene (C(1)–C(6)–C(7)–C(8)–C(9)–N(1)) are approximately perpendicular with a dihedral angle of 106.5°. The crystal packing of the compound was stabilized by two weak interactions between C(11)–H(10)···N(4) and C(18)–H(18)···N(1). In fluorescence spectra studies, compound 4c was exhibited good selectivity and sensitivity towards iron ions in DMSO: mops buffer solution. Furthermore, 4c was successfully used for imaging iron ions in living HeLa cervical cancer cells.