Xueting Hu, Lijia Zhao*, Tingting Liao, Cheng-Peng Li*

Cite this article: Chin. J. Struct. Chem., 2026, 45(2), 100793. DOI: 10.1016/j.cjsc.2025.100793

Publication date: February 1, 2026

Keywords: Crystalline porous organic frameworks; Hydrogen-bonded organic frameworks; Covalent organic frameworks; Enzyme; Catalyst; Biomedicine application

ABSTRACT

Crystalline porous organic frameworks (CPOFs), with their highly ordered pores and tunable organic structures, have shown immense promise as platforms for enzyme immobilization. However, research on enzyme@CPOF composites, particularly for biomedical applications, is still in its early stages and lacks comprehensive and systematic review. This article provides a thorough overview of recent advances in the rational design, synthesis, and application of enzyme@CPOF biocomposites. Emphasis is placed on immobilization strategies and the structure-performance relationships revealed through molecular-level investigations. Furthermore, we highlight emerging applications in biocatalysis and biomedical engineering, and discuss persistent challenges and future directions to advance CPOFs as versatile, high-performance substrates for enzyme immobilization.